Identification and Typing of Aeromonas Hydrophila through 16S rDNA-PCR Fingerprinting

Raghava et al. stated that the identification and classification of microorganisms are of fundamental importance in microbiology [2]. Classical methods are basically dependent on phenotypic data. These are also time consuming and expensive. Various fingerprinting methods can be broadly classified DNA fingerprinting by restriction enzyme analysis of the genomic DNA on pulse field gel electrophoresis, for a particular region of the genome the repetitive DNA sequence can be used as a probe, DNA-DNA hybridization and amplification of particular region of genome by PCR followed by restriction analysis. All of these methods of genomic DNA have been the subject of analysis (Grimont and Grimont [3], Huys et al. [4] and Skuce et al. [5]). A. hydrophila may exhibit slightly different characteristics depending on the environment from which they were isolated. However, all of these methods have been used for epidemiological studies or to differentiate between strains of the same species or to confirm the species of the genus.